Peroxisome proliferator-activated receptors (Ppars) : methods and protocols /
edited by Mostafa Z. Badr, Jihan Youssef.
- New York : Humana Press ; Springer, c2013.
- xi, 343 p. : ill. (some col.) ; 26 cm.
- Methods in molecular biology, 952 1064-3745 ; Springer protocols .
- Methods in molecular biology (Clifton, N.J.) ; v. 952. Springer protocols. .
Includes bibliographical references and index.
PPARs : history and advances / PPAR-alpha cloning, expression, and characterization / PPARGC1A and PPARG genotyping : beginner's guide to genotyping with unlabeled probes / Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR [beta]/[delta] overexpression / Specific knockdown of PPAR[delta] gene in colon cancer cells by lentivirus-mediated RNA Interfering / Dominant-negative and knockdown approaches to studying PPAR activity / Producing PPARgamma2 knockdown in mouse liver / Adipose tissue-specific PPAR[gamma] gene targeting / Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs / PPAR SUMOylation : some useful experimental tips / Analyzing phosphorylation-dependent regulation of subcellular localization and transcriptional activity of transcriptional coactivator NT-PGC-1[alpha] / In vivo studies of PPAR-chromatin interactions : chromatin immunoprecipitation for single-locus and genomewide analyses / FISH analysis using PPAR[gamma]-specific probes for detection of PAX8-PPAR[gamma] translocation in follicular thyroid neoplasms / Immunohistochemical techniques to identify and localize proteins of interest in paraffin embedded tissue sections / Determination of PPAR expression by western blot / Fluorescence resonance energy transfer techniques to study ligand-mediated interactions of PPARs with coregulators / Estimation of the PPAR[alpha] agonism of fibrates by a combined MM-docking approach / Combined biophysical and cell-based approaches for the assessment of ligand binding to PPAR[gamma] / Exploring PPAR modulation in experimental mice / Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold / Analyzing PPAR[alpha]/ligand interactions by chemical cross-linking and high-resolution mass spectrometry / Synthesis, mass spectrometric characterization, and analysis of the PPAR[delta] agonist GW1516 and its major human metabolites : targets in sports drug testing / LC-MS-based method for the qualitative and quantitative analysis of the novel PPAR[gamma] agonist KR-62980 / Behavioral paradigms to evaluate PPAR modulation in animal models of brain injury / Jihan Youssef and Mostafa Z. Badr -- Suong N.T. Ngo and Ross A. McKinnon -- Viera Habalova and Jozef Židzik -- Teayoun Kim ... [et al.] -- Lie Yang, Zong-Guang Zhou, and Xiao-Feng Sun -- Sudhir Singh and Robert G. Bennett -- Tomomi Yamazaki and Osamu Ezaki -- Weimin He -- Nico Mitro ... [et al.] -- Benoit Pourcet, Bart Staels, and Corine Glineur -- Ji Suk Chang and Thomas W. Gettys -- Till Adhikary and Rolf Müller -- Noor Akmal Syed Husain Sharifah, Zubaidah Zakaria, and Wai Kit Chia -- Carolyn M. Komar and Mary Jane Long -- Aziz Guellich and Catherine Coirault -- Nico Mitro, Cristina Godio, and Maurizio Crestani -- Fabio Lannutti, Alessandro Marrone, and Nazzareno Re -- Silvia Zorrilla and Dolores Pérez-Sala -- Anna Rull ... [et al.] -- Yoshitaka Miyagawa, Hajime Okita, and Nobutaka Kiyokawa -- Mathias Q. Müller, Christian H. Ihling, and Andrea Sinz -- Mario Thevis ... [et al.] -- Min-Sun Kim and Myung Ae Bae -- Dana Greene-Schloesser, Caroline I. Schnegg, and Mike E. Robbins.