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Peroxisome proliferator-activated receptors (Ppars) : methods and protocols / edited by Mostafa Z. Badr, Jihan Youssef.

Mitwirkende(r): Badr, Mostafa Z, 1950- [edt] | Youssef, Jihan A, 1959- [edt].
Materialtyp: materialTypeLabelBuchReihen: Methods in molecular biology (Clifton, N.J.): v. 952.; Springer protocols: Verlag: New York : Humana Press ; Springer, c2013Beschreibung: xi, 343 p. : ill. (some col.) ; 26 cm.ISBN: 9781627031547 (alk. paper); 1627031545 (alk. paper).Schlagwörter: Peroxisomes | Nuclear receptors (Biochemistry) | Peroxisome Proliferator-Activated Receptors -- Laboratory ManualsDDC-Klassifikation: 572.8
Inhalte:
PPARs : history and advances / Jihan Youssef and Mostafa Z. Badr -- PPAR-alpha cloning, expression, and characterization / Suong N.T. Ngo and Ross A. McKinnon -- PPARGC1A and PPARG genotyping : beginner's guide to genotyping with unlabeled probes / Viera Habalova and Jozef Židzik -- Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR [beta]/[delta] overexpression / Teayoun Kim ... [et al.] -- Specific knockdown of PPAR[delta] gene in colon cancer cells by lentivirus-mediated RNA Interfering / Lie Yang, Zong-Guang Zhou, and Xiao-Feng Sun -- Dominant-negative and knockdown approaches to studying PPAR activity / Sudhir Singh and Robert G. Bennett -- Producing PPARgamma2 knockdown in mouse liver / Tomomi Yamazaki and Osamu Ezaki -- Adipose tissue-specific PPAR[gamma] gene targeting / Weimin He -- Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs / Nico Mitro ... [et al.] -- PPAR SUMOylation : some useful experimental tips / Benoit Pourcet, Bart Staels, and Corine Glineur -- Analyzing phosphorylation-dependent regulation of subcellular localization and transcriptional activity of transcriptional coactivator NT-PGC-1[alpha] / Ji Suk Chang and Thomas W. Gettys -- In vivo studies of PPAR-chromatin interactions : chromatin immunoprecipitation for single-locus and genomewide analyses / Till Adhikary and Rolf Müller -- FISH analysis using PPAR[gamma]-specific probes for detection of PAX8-PPAR[gamma] translocation in follicular thyroid neoplasms / Noor Akmal Syed Husain Sharifah, Zubaidah Zakaria, and Wai Kit Chia -- Immunohistochemical techniques to identify and localize proteins of interest in paraffin embedded tissue sections / Carolyn M. Komar and Mary Jane Long -- Determination of PPAR expression by western blot / Aziz Guellich and Catherine Coirault -- Fluorescence resonance energy transfer techniques to study ligand-mediated interactions of PPARs with coregulators / Nico Mitro, Cristina Godio, and Maurizio Crestani -- Estimation of the PPAR[alpha] agonism of fibrates by a combined MM-docking approach / Fabio Lannutti, Alessandro Marrone, and Nazzareno Re -- Combined biophysical and cell-based approaches for the assessment of ligand binding to PPAR[gamma] / Silvia Zorrilla and Dolores Pérez-Sala -- Exploring PPAR modulation in experimental mice / Anna Rull ... [et al.] -- Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold / Yoshitaka Miyagawa, Hajime Okita, and Nobutaka Kiyokawa -- Analyzing PPAR[alpha]/ligand interactions by chemical cross-linking and high-resolution mass spectrometry / Mathias Q. Müller, Christian H. Ihling, and Andrea Sinz -- Synthesis, mass spectrometric characterization, and analysis of the PPAR[delta] agonist GW1516 and its major human metabolites : targets in sports drug testing / Mario Thevis ... [et al.] -- LC-MS-based method for the qualitative and quantitative analysis of the novel PPAR[gamma] agonist KR-62980 / Min-Sun Kim and Myung Ae Bae -- Behavioral paradigms to evaluate PPAR modulation in animal models of brain injury / Dana Greene-Schloesser, Caroline I. Schnegg, and Mike E. Robbins.
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Includes bibliographical references and index.

PPARs : history and advances / Jihan Youssef and Mostafa Z. Badr -- PPAR-alpha cloning, expression, and characterization / Suong N.T. Ngo and Ross A. McKinnon -- PPARGC1A and PPARG genotyping : beginner's guide to genotyping with unlabeled probes / Viera Habalova and Jozef Židzik -- Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR [beta]/[delta] overexpression / Teayoun Kim ... [et al.] -- Specific knockdown of PPAR[delta] gene in colon cancer cells by lentivirus-mediated RNA Interfering / Lie Yang, Zong-Guang Zhou, and Xiao-Feng Sun -- Dominant-negative and knockdown approaches to studying PPAR activity / Sudhir Singh and Robert G. Bennett -- Producing PPARgamma2 knockdown in mouse liver / Tomomi Yamazaki and Osamu Ezaki -- Adipose tissue-specific PPAR[gamma] gene targeting / Weimin He -- Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs / Nico Mitro ... [et al.] -- PPAR SUMOylation : some useful experimental tips / Benoit Pourcet, Bart Staels, and Corine Glineur -- Analyzing phosphorylation-dependent regulation of subcellular localization and transcriptional activity of transcriptional coactivator NT-PGC-1[alpha] / Ji Suk Chang and Thomas W. Gettys -- In vivo studies of PPAR-chromatin interactions : chromatin immunoprecipitation for single-locus and genomewide analyses / Till Adhikary and Rolf Müller -- FISH analysis using PPAR[gamma]-specific probes for detection of PAX8-PPAR[gamma] translocation in follicular thyroid neoplasms / Noor Akmal Syed Husain Sharifah, Zubaidah Zakaria, and Wai Kit Chia -- Immunohistochemical techniques to identify and localize proteins of interest in paraffin embedded tissue sections / Carolyn M. Komar and Mary Jane Long -- Determination of PPAR expression by western blot / Aziz Guellich and Catherine Coirault -- Fluorescence resonance energy transfer techniques to study ligand-mediated interactions of PPARs with coregulators / Nico Mitro, Cristina Godio, and Maurizio Crestani -- Estimation of the PPAR[alpha] agonism of fibrates by a combined MM-docking approach / Fabio Lannutti, Alessandro Marrone, and Nazzareno Re -- Combined biophysical and cell-based approaches for the assessment of ligand binding to PPAR[gamma] / Silvia Zorrilla and Dolores Pérez-Sala -- Exploring PPAR modulation in experimental mice / Anna Rull ... [et al.] -- Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold / Yoshitaka Miyagawa, Hajime Okita, and Nobutaka Kiyokawa -- Analyzing PPAR[alpha]/ligand interactions by chemical cross-linking and high-resolution mass spectrometry / Mathias Q. Müller, Christian H. Ihling, and Andrea Sinz -- Synthesis, mass spectrometric characterization, and analysis of the PPAR[delta] agonist GW1516 and its major human metabolites : targets in sports drug testing / Mario Thevis ... [et al.] -- LC-MS-based method for the qualitative and quantitative analysis of the novel PPAR[gamma] agonist KR-62980 / Min-Sun Kim and Myung Ae Bae -- Behavioral paradigms to evaluate PPAR modulation in animal models of brain injury / Dana Greene-Schloesser, Caroline I. Schnegg, and Mike E. Robbins.

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