000			03049nam a22002177a 4500
999			_c59336 _d59336
008			180912s2017 ua ||||g b||| 00| 0 eng d
040			_cSOUL
041			_aara _heng
082	4	0	_221 _a572 _bM P
100			_94526 _aWilliam, Mira Magdy.
245	0	1	_a Potential Protective Effects of Avocado Extract (Persea americana) Against Some Chemotherapeutic Agents / _cMira Magdy William ; Supervised by Tarek M. K. Motawi, Hanan M. Abdelgawad, Mohammed M. Nooh.
246	0	3	_aالآثار الوقائية المحتملة لمستخلص الأفوكادو ضد بعض عقاقير العلاج الكيميائي
260			_aCairo : _bCairo University _c2017.
300			_avii, 3, 159 p. : _c25 cm. _e+CD
500			_aThesis (M.Sc.) – Cairo University. Faculty of Pharmacy. Department of Biochemistry.
504			_aIncludes bibliographical references.
520			_aUse of cyclophosphamide (CPA) or fludarabine as chemotherapeutic agents is associated with deleterious side effects including pulmonary toxicity and urotoxicity or myelosuppression, respectively. Avocado, Persea americana Mill. is a widely consumed fruit in many countries for its nutritional and medicinal benefits. In the present study, we investigated the modulating effect of avocado fruit extract (AE) on some metabolizing enzymes involved in the activation and detoxification of CPA and fludarabine. Three phases of CPA-induced urotoxicity and pulmonary toxicity were studied; 2, 7 and 28 days after a single i.p. injection at a dose of 150 mg/kg. Fludarabine was injected (5 mg/kg/day, i.p.) for five consecutive days and tested for its myelosuppression 12 days post first injection. Cyclophosphamide induced acute elevation followed by late reduction in cytochrome P450 2B6 (CYP2B6) level in hepatic microsomes and significantly suppressed bladder and lung glutathione-S-transferase (GST) activity. Furthermore, CPA elevated bladder blood content, lung myeloperoxidase activity, DNA and hydroxyproline contents. Fludarabine produced a significant decrease in deoxycytidine kinase (dCK) and cytosolic nucleotidase II (cN-II) levels. In addition, fludarabine administration reduced total leukocytic and lymphocytic count as well as DNA polymerase and ribonucleotide reductase levels. Avocado extract administration (900 mg/kg/day p.o.) for 7 days prior to CPA and fludarabine exposure and its continuation for 2, 7 and 28 days post CPA and 12 days post first fludarabine injection, ameliorated all CPA and fludarabine induced derangements in spite of variable effects on metabolizing enzymes. Avocado extract administration suppressed CYP2B6 level, augmented GST activity of CPA treated rats and restored the level of both dCK and cN-II of fludarabine treated rats. In conclusion, these results show that AE plays a protective role against CPA and fludarabine-induced toxicity by regulating drug metabolizing enzymes and exerting antioxidant activity.
650	4	0	_91617 _aBiochemistry.
700			_94527 _aNooh, Mohammed M. _eSupervisor
942			_2ddc _cTHE _iFOP _6FOP